A recent years. They have evolved into analysis of tumor Abstract— Multiplexed platforms have become a standard feature of modern medicine in the field of histopathology in recent years. They have evolved into powerful technologies that enable image analysis of tumor tissues from formalin- fixed paraffin- embedded specimens, aiming for better assessment of morphology and distinctive alterations at the molecular level of the patient’s sample, which is critical for the pathologist’s diagnosis and classification, with significant implications for the following therapeutic options. And also, in order to gain a better understanding of the tumor microenvironment, which aids cancer prevention by simulating new therapy discoveries. And unlike traditional IHC, which can only identify one marker in a tissue sample, multiplex IHC may detect many markers in a single tissue sample while providing detailed information about the cell composition and spatial arrangement. Reviewing multiplexed technologies is to demonstrate their utility in the study of cancer tissue as well as their benefits for applications in cancer diagnosis, stratifying patients, and accuracy for treatment. Summary: Digital pathology plays a significant part in current clinical practice and becoming an increasingly critical technological necessity in the laboratory environment, Algorithms for image analysis and artificial intelligence have the potential to further increase the quality of diagnostics in pathology. Keywords— Digital pathology; Molecular diagnostics; IHC & MIHC Techniques; cancers. 

Abstract

Gall bladder cancers, predominantly adenocarcinomas, are associated with significant malignancy and are more common in women, especially in their seventh decade of life. This study aims to evaluate the prevalence, distribution, and histopathological characteristics of gall bladder tumors, emphasizing the need for early diagnosis and understanding the role of chronic cholecystitis in tumor development. A retrospective analysis was performed on 45740 surgical biopsies, including 3107 cholecystectomies, from 1994 to 2007 at Misurata Medical Center, Libya. Data collected included patient demographics, clinical details, and microscopic diagnoses. Biopsy samples were processed and stained, and histopathological evaluation was performed following standard criteria. The study showed a female predominance in gall bladder tumors with a female: male ratio of 3:1. Most tumors were adenocarcinomas (83.33%), with high and moderate differentiation being the most frequent. Tumor diagnoses were most prevalent in the 51-65 age group. The majority of tumors were diagnosed at advanced stages (3 and 4). Tumor invasion was common, with 75% showing perivascular, intravascular, or perineural involvement. Chronic cholecystitis was observed in more than two-thirds of the tumor cases. Chronic inflammation is closely linked to gallbladder carcinogenesis, with most tumors diagnosed at advanced stages due to vague symptoms and aggressive behavior. Early detection and multidisciplinary approaches are vital to improve outcomes, especially in high-risk groups.

Abstract

Microscopic diagnosis of gastrointestinal biopsies remains a challenge in surgical pathology, involving both neoplastic and non-neoplastic changes. This study aims to analyze the prevalence, histopathological features, and grading of gastrointestinal (GIT) tumors in biopsies collected over 12.5 years at Misurata Medical Center, Libya, while assessing demographic patterns and the correlation of Helicobacter pylori infection with gastric malignancies. A total of 753 GIT biopsies were retrospectively analyzed from 1995 to 2007. Clinical data, including age, sex, and microscopic diagnoses, were documented. Biopsies underwent histopathological evaluation using the World Health Organization (WHO) classification. Statistical analyses were performed using SPSS software to explore relationships between neoplastic lesions and demographic factors. Non-neoplastic diseases accounted for 559 cases, which constitute 74.24% of the total cases, while neoplastic lesions were identified in 194 cases (25.76%). with a statistically significant predominance of malignant tumors (68.6%) over benign ones (31.4%, p<0.001). A slight male predominance was observed in overall biopsies (52.2%), with the 61-70 age group showing the highest frequency (17.4%). Well-differentiated tumors were more common, correlating with better prognoses. The TNM staging analysis revealed a concerning trend toward late-stage diagnoses, particularly in stomach and colorectal cancers. The investigation into Helicobacter pylori positivity indicated a complex relationship with gastric malignancies, warranting further research. The findings reflect a high burden of GIT malignancies during the study period, with late-stage diagnosis and limited diagnostic resources. These results underscore the need for improved screening programs, early detection efforts, and enhanced diagnostic infrastructure in Libya. The study underscores the importance of understanding the multifactorial influences of Helicobacter pylori in gastric cancer development.

This study aimed to assess surface contamination levels of children’s playground equipment in two public parks in AL-Zawiya City, Libya (Jdayem Park and AL-Zawiya Park), using adenosine triphosphate (ATP) bioluminescence monitoring. The equipment surveyed included plastic slides, metal slides, iron swings, plastic swings, and rope swings. A total of 24 samples were collected from these surfaces using standardized ATP swabs, and results were classified based on established RLU thresholds (<100 RLU: clean, 100–300 RLU: marginally contaminated, >300 RLU: contaminated). The results revealed significant variability across equipment and surface types. The highest contamination level was observed on the iron swing at Jdayem Park (21–416 RLU), while the lowest levels were recorded on rope swings in AL-Zawiya Park (11–25 RLU). Plastic surfaces and rope swings generally exhibited lower contamination compared to metal surfaces, suggesting that surface material and design, combined with usage frequency, play a pivotal role in contamination accumulation. These findings align with previous studies that highlight the role of surface characteristics and environmental exposure in influencing microbial load. The results underscore the urgent need for targeted cleaning, disinfection, and regular quality

.monitoring of public playground equipment to maintain a safe, hygienic, and child-friendly recreational environment

In this study, the morphological and thermal properties of a blend consisting of 75% polyvinyl chloride (PVC) and

25% low-density polyethylene (LDPE), reinforced with nano-refractory bricks (NRB) at varying ratios (1%, 3%, 5%, and 7%),

were prepared and investigated. The objective was to enhance the blend’s thermal stability and surface structure homogeneity.

The morphological structure of the prepared samples was analyzed using scanning electron microscopy (SEM) and X-ray

diffraction (XRD). The obtained images revealed that the nano-refractory brick particles remained predominantly within the

PVC phase, resulting in an increased composite density without significantly affecting the LDPE phase. This suggests that the

overall morphology of the composite is largely unaffected by the presence of the nano-refractory bricks. From a thermal

perspective, thermogravimetric analysis (TGA/DTG) was conducted on all samples from room temperature up to 600 °C at a

heating rate of 10 °C/min. FTIR and TGA results indicated that the decomposition onset temperature shifted to higher values

due to the presence of nano-refractory bricks, with the most pronounced shift observed in the sample containing 3%

reinforcement. Additionally, the rate of mass loss was reduced. These findings demonstrate that reinforcing the PVC/LDPE

blend with nano-refractory bricks improves its performance, making it more suitable for applications that demand enhanced

thermal and mechanical properties, such as those in the construction and electronics industries.